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söndag 12 april 2026

Uusinta Tn antigeenista ja syövästä. Fokus Kaitsijaproteiiniin COSM ja aktiiviin T-syntaasiin, entsymaattiseen O-glykosylaatioon.

 

 
GALNT1 emerges as a potential therapeutic target in breast cancer.
Li J, Zhong P, Li X, Huang R, Zhan Z, Li J, Mao M.J Cancer Res Clin Oncol. 2026 Mar 25;152(3):74. doi: 10.1007/s00432-026-06448-2.PMID: 41880012 Free PMC article.
RESULTS: GALNT1 was significantly upregulated in primary breast cancer as well as multiple other malignancies. In vitro experiments demonstrated that GALNT1 promoted breast cancer cell proliferation, migration, and O-glycosylation. Immunofluorescence staining reveal …
 
Transforming Growth Factor-beta Drives Epithelial Mesenchymal Transition and Reduces Synthesis of Unelaborated O-GalNAc Glycans in Breast Cancer Cells.
Cull J, Pink RC, Samuel P, Brooks SA.J Histochem Cytochem. 2026 Mar-Apr;74(2):193-214. doi: 10.1369/00221554261416994. Epub 2026 Feb 28.PMID: 41764140 Free PMC article.
The effect of TGF-beta1 on synthesis of unelaborated Tn antigen was explored using Helix pomatia agglutinin (HPA) labelling. TGF-beta1 treatment induced morphological changes, resulting in an elongated, mesenchymal-like phenotype, and a reduction of epithelial marke …
 
Deletion of Core 1 β3GalT-specific molecular chaperone (Cosmc) in murine intestinal epithelia leads to major alterations in glycocalyx and tumorigenesis.
Ju T, Wang Y, Nishio H, Kudelka MR, Sun X, Wang J, Zeng J, Song L, Akkas G, Adsay V, Parkos CA, Cummings RD.J Biol Chem. 2026 Feb 25;302(4):111319. doi: 10.1016/j.jbc.2026.111319. Online ahead of print.PMID: 41759737 Free PMC article.
Expression of such glycans is under control of Cosmc (C1GalT1C1) that encodes a key ER molecular chaperone required for formation of active T-synthase, a Golgi enzyme that modifies the Tn antigen (GalNAcalpha1-Ser/Thr/Tyr - CD175) to generate a Core 1 O-glycan. We p …
 
Liposome-Based Potential Vaccines Platforms that Are Noncytotoxic.
Mebarek S, Jacob K, Pierro CI, Romanini D, Fiore M.ChemistryOpen. 2026 Jan;15(1):e202500530. doi: 10.1002/open.202500530.PMID: 41521398 Free PMC article.
Synthetic vaccines represent a promising avenue in cancer immunotherapy by promoting targeted immune responses. Liposomal technologies have further advanced synthetic vaccinology by enabling the efficient delivery of tumor-associated carbohydrate antigens. ...In this preli …
 
Higher-Energy Collisional Dissociation Mass Spectrometry Fragmentation Enables Distinguishing O-GlcNAc from Tn Antigen in Cancer Cells.
Chen Q, Cheng W, Li C, Fung YME, Wang J, Liu H, Li X.J Am Soc Mass Spectrom. 2026 Feb 4;37(2):491-504. doi: 10.1021/jasms.5c00378. Epub 2026 Jan 10.PMID: 41518202
One representative example is O-linked beta-N-acetylglucosamine (O-GlcNAc) and O-linked alpha-N-acetylgalactosamine (Tn antigen). Traditional biochemistry approaches used in separating O-GlcNAc- and Tn antigen-modified peptides mainly include chemical …
 
Diagnostic Potential of Tn-MUC1 in Breast Cancer: A Novel Immunohistochemical Marker Reflecting Tumor Progression.
Shimizu A, Hatanaka KC, Nange A, Okumura A, Takehashi M, Shinomiya Y, Naruchi K, Sato M, Kase H, Mitsuhashi T, Yamashita H, Hatanaka Y, Matsuno Y.Lab Invest. 2026 Mar;106(3):106070. doi: 10.1016/j.labinv.2025.106070. Epub 2026 Jan 6.PMID: 41506350
Recently, a novel epitope-defined antibody (MUC1-Tn antigen epitope-defined antibody [MUC1-Tn ED Ab]) that specifically recognizes the Tn-MUC1 antigen was developed. ...In multilesion analysis, cytoplasmic Tn-MUC1 expression was rarely detected in nonneoplastic area …
 
A novel method for Tn antigen detection based on chemoenzymatic targeted labelling and CuAAC click chemistry.
Duan H, Liu L, Guo X, Wang Z, Fang Z, Huang H, Zhang H, Li Y, Wang Y, Ye M, Jia L, Dong M.Chem Commun (Camb). 2026 Jan 29;62(8):2599-2602. doi: 10.1039/d5cc05398f.PMID: 41496556
The Tn antigen is a key tumor-associated antigen and a promising cancer biomarker. However, its low abundance poses great challenges for sensitive detection. We developed a novel approach that integrates B3GNT6-mediated Tn-specific labelling, click chemistry, …
 
Synthetic Multivalent Tn Antigen Elicits Valency-Dependent IgG Antibody Production.
Thekke Veettil K, Jayaraman N.Bioconjug Chem. 2026 Jan 21;37(1):20-30. doi: 10.1021/acs.bioconjchem.5c00444. Epub 2025 Dec 16.PMID: 41401309
The Tn antigen is an attractive target for cancer vaccine development. In this study, we report the efficacies of dendrimer-based multivalent Tn antigens to elicit profound antibody generation in mice, in a subtype-specific manner. Multivalency of the Tn …
 
Tumor-associated Tn and STn antigens: from molecular mechanism to precision diagnosis and treatment.
Zhao S, Fu C, Gong B, Wu H, Zhang R, Cai H, Chen H.Front Immunol. 2025 Nov 26;16:1660852. doi: 10.3389/fimmu.2025.1660852. eCollection 2025.PMID: 41383589 Free PMC article. Review.
BACKGROUND: Abnormal protein glycosylation is a key feature of tumors. Among the modifications, Tn antigen (GalNAcalpha1-Ser/Thr) and its sialylated derivative, STn antigen (Neu5Acalpha2-6GalNAcalpha1-O-Ser/Thr), are prominent tumor-associated carbohydrate antigens. …

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